Amino Acid Chemistry

Note: These are incomplete and I have no intention of completing them.

Amino Acid Chemistry Table of Contents

Sequencing Proteins

Composition

Amino Acid Modifications

part3

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Edman Degredation

• Automated reaction, N to C
• Sequential, limited to 20-50 residues

Fragmentation

• Random
  • Proteases
  • Acid
• Specific
  • CNBr

C-terminus Sequencing

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• Guanamine Hydrochloride degrades amino acids, but also convers Asn, Gln to Asp, Glu, and Trp, Cys, and Tyr are entirely degraded. However, this method is useful to identify modified amino acids.
• Mass spectrometry can measure the mass of a protein very accurately. It is limited to proteins of 100kD or less due to problems with getting them into the gas phase.

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